The major scientific and technological projects in the dairy industry have achieved breakthroughs in the techniques of cow sex identification, XY sperm isolation, live egg collection, and in vitro fertilization through research efforts by researchers. The success rate of gender identification for dairy cows reaches 98%. Using live egg collection technology, each donor cow can obtain 3 to 5 eggs per week. A stable and effective bovine oocyte in vitro fertilization solution system and in vitro fertilized embryos are established. In vitro culture technology system, the development rate of in vitro fertilized blastocysts was stabilized at about 47%; after in vitro maturation, in vitro fertilization and in vitro culture, the oocyte maturation rate, fertilization rate and embryo development rate reached 85% and 70% respectively. And 25%; the use of flow cytometry to separate X, Y sperm, per second can be separated from 1.5 to 20,000 sperm. Applying the above core technologies to establish a technology system for sexually controlled embryonic industrial production. At present, three dairy cow embryo centers have been established to form an annual output of more than 20,000 high-quality and high-yield dairy cow embryos, accounting for more than 50% of the current domestic dairy cow embryo market demand. So far it has produced more than 26,000 high-quality embryos, has transplanted more than 16,000 head receptors, and the average pregnancy rate has reached 53.3%. The level of technology and industrialization have reached the international advanced level, solving the problem of rapid expansion of dairy cows and rapid expansion. The foundation of the core group of elite cows in China has been laid.
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