Genetic development scientists identify self-incompatible pollen factors

The innovation team led by the researcher Xue Yongxuan of the Institute of Genetic Development made a breakthrough in the research on the molecular mechanism of plant sexual reproduction and self-incompatibility, and established the self-incompatible pollen factor. Its research results were published on the famous journal THE PLANT CELL. . The magazine also wrote a review article for this purpose (Eckardt, 2004, Plant Cell 16: 558-561). This achievement is one of the major achievements in the field of sexual reproduction research in international plants in the past decade. In order to avoid inbreeding, plants have developed a self-incompatibility mechanism during long-term evolution to distinguish pollen from itself or not. In self-incompatible plants, the stigma of the mother can recognize pollen that she owns or comes from other plants but has the same genotype as the mother, thus inhibiting the germination and growth of the pollen, and ultimately cannot be fertilized. However, pollen from different strains and with different genotypes of the mother is not affected and can germinate and fertilize. In nature, there are two types of self-incompatibility: spore-determined and gamete-determined. In the spore-determined form, whether or not the affinity depends on the genotype of the mother and the father rather than the pollen. Conversely, in the gamete-determined type, whether the affinity is determined by the genotype of the mother and the pollen is not related to the paternal genotype. In the early 1990s, it was found that in the gamete-determined form, the determinant of the maternal body is S-RNase, but its mechanism of action and pollen determinants are unclear. In the long-term research and exploration, the innovation team led by Dr. Xue Yongzheng first cloned a gene related to the pollen determinant factor AhSLF-S2 (Lai et al., 2002, Plant Mol Biol 50:29-42) and proved the gene. Genetic characteristics with pollen determinants (Zhou et al., 2003, Sex Plant Reprod 16:165-177). The group's Qiao Hong and other students in turn transferred AhSLF-S2 to the self-incompatible petunia, proving that it can turn self-incompatible petunias into affinity, which proves that AhSLF-S2 It is indeed a self-incompatible pollen determinant (Qiao et al., 2004, Plant Cell, 16:2307-2322) that solves a very common problem in botany. How AhSLF-S2 interacts with the female factor S-RNase, leading to self-incompatibility. Qiao Hong and others used techniques such as co-immunoprecipitation and yeast two-hybridization to demonstrate for the first time that AhSLF-S2 and S-RNase and SCF proteins The ASK1, CULLIN interactions in the degraded complexes, and through specific inhibitors and biochemical assays, demonstrate that S-RNase is ubiquitinated in affinity combinations (Qiao et al., 2004, Plant Cell 16:582-595). The innovation of this study is to demonstrate for the first time the key role of AhSLF-S2 in self-incompatibility and to prove that the degradation of S-RNase is achieved through the AhSLF-S2-mediated ubiquitin/26S proteolytic degradation pathway. . The research results have solved the pending pollen determinants in self-incompatibility and provided the conditions for understanding genetic, evolutionary and crop genetic breeding of self-incompatibility mechanisms. At the same time, it is also the most original and influential work for scientists in the field of plant science after the Chinese scientists studied rice genes.

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